Peran Mini-Barcode Internal Transcribed Spacer 2 (ITS2) untuk Identifikasi Molekuler Spesies Anggrek

AbstractMolecular identification using short orthological DNA sequences (DNA barcoding) has been applied for the classification of orchid species which is a major step in biodiversity management, conservation, breeding, authenticating components herbal products, and tracking adulteration species. One most widely used loci phylogenetic inference at generic infrageneric levels plants was ITS located between 18S rDNA, 5.8S 26S rDNA. The or ITS2 region suggested as plant barcode some previous studies. However, barcoding entire genome considered less effective efficient process PCR amplification sequencing. Besides, complete barcodes are difficult to obtain from herbarium samples products because have degraded. mini-barcodes were developed over past ten years overcome issues related barcoding. use shorter segments amplification, so they can identify effectively efficiently compared regular Specific primers that encode ITS1 regions need be designed amplification. mini-barcoding proven useful identification, studies, authentication specific Therefore, new rapid method based on mini-barcode expected established, especially Keywords : mini-barcode, Internal Transcribed Spacer, Orchid, Primer Design